Chk2 (human, recombinant)

Checkpoint kinase 2 (Chk2) is a serine/threonine kinase and downstream effector of ataxia-telangiectasia mutated kinase (ATM) in the cellular DNA damage response. It is composed of an N-terminal serine-glutamine/threonine-glutamine cluster domain (SCD), a central forkhead-associated (FHA) domain, and a C-terminal serine/threonine kinase domain. Chk2 is ubiquitously expressed and localized to the nucleus. In response to double stranded breaks (DSBs) generated by genotoxins or radiation, Chk2 is phosphorylated and activated by ATM, inducing cell cycle arrest at the S or G2/M phase. Chk2 also has roles in various mitotic processes, including spindle formation, cytokinesis, checkpoint signaling, and chromosome segregation. Overexpression of Chek2 is associated with disease progression in papillary thyroid cancer. Cayman's Chk2 (human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications. This protein has a calculated molecular weight of 88 kDa.