Chaperonin GroEL (groEL), Chlamydia trachomatis, recombinant

Organism: Chlamydia trachomatis (strain D/UW-3/Cx). Source: E.coli. Expression Region: 2-544aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: VAKNIKYNEE ARKKIQKGVK TLAEAVKVTL GPKGRHVVID KSFGSPQVTK DGVTVAKEVE LADKHENMGA QMVKEVASKT ADKAGDGTTT ATVLAEAIYT EGLRNVTAGA NPMDLKRGID KAVKVVVDQI RKISKPVQHH KEIAQVATIS ANNDAEIGNL IAEAMEKVGK NGSITVEEAK GFETVLDIVE GMNFNRGYLS SYFATNPETQ ECVLEDALVL IYDKKISGIK DFLPVLQQVA ESGRPLLIIA EDIEGEALAT LVVNRIRGGF RVCAVKAPGF GDRRKAMLED IAILTGGQLI SEELGMKLEN ANLAMLGKAK KVIVSKEDTT IVEGMGEKEA LEARCESIKK QIEDSSSDYD KEKLQERLAK LSGGVAVIRV GAATEIEMKE KKDRVDDAQH ATIAAVEEGI LPGGGTALIR CIPTLEAFLP MLTNEDEQIG ARIVLKALSA PLKQIAANAG KEGAIIFQQV MSRSANEGYD ALRDAYTDML EAGILDPAKV TRSALESAAS VAGLLLTTEA LIAEIPEEKP AAAPAMPGAG MDY. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity). This protein is implicated in the pathogenesis of chlamydial disease. Inflammation elicited by the 57KDA antigen may damage tissue, with progression to scarring of conjunctival and fallopian tube mucosae, which respectively result in blindness and infertility. Reference: "Genome sequence of an obligate intracellular pathogen of humans: Chlamydia trachomatis."Stephens R.S., Kalman S., Lammel C.J., Fan J., Marathe R., Aravind L., Mitchell W.P., Olinger L., Tatusov R.L., Zhao Q., Koonin E.V., Davis R.W.Science 282:754-759(1998). Function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions (By similarity). This protein is implicated in the pathogenesis of chlamydial disease. Inflammation elicited by the 57 kDa antigen may damage tissue, with progression to scarring of conjunctival and fallopian tube mucosae, which respectively result in blindness and infertility.