Cellulase (Onozuka R-10)

Cellulase (Onozuka R-10) is a multi-component enzyme system derived from Trichoderma viride and contains cellulase, alpha-amylase, hemicellulase, pectinase and protease activities. It is often used in the isolation of plant protoplasts in combination with other enzymes. Source: Trichoderma viride, Synonyms: Onozuka R-10, CAS No: 9012-54-8, EC Number: 3.2.1.4, Unit Definition of Activity: One unit of Cellulase will liberate 1.0 micromole of glucose form carboxymethyl cellulose per minute at 40 °C, pH 4.5, glucose determined with alkaline copper reagent (3). Major Enzyme Activity: Cellulase: 1U/mg, Extraneous Enzyme Activities: Pectinase: 0.4U/mg, Hemicellulase: 1.0U/mg, alpha-Amylase: 0.6U/mg, Protease: 0.01U/mg, Optimum pH range: 4-5, Solubility: Soluble in water (10mg/ml), Loss on Drying: <10%, Arsenic: <3ug, Heavy Metals: <0.0005%, Activity: >10,000U/g, Total Viable Count: <10,000cfu/g, Fungi: <100cfu/g, Coliform: Negative, Stability and Storage: Lyophilized powder may be stored at 4°C. Stable for 12 months at 4°C. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards:  All products should be handled by qualified personnel only, trained in laboratory procedures.