Cellulase 9K, Ruminiclostridium thermocellum

Cellulase 9K (RtCel9K), assigned the E.C. number 3.2.1.4, is a derivative of Ruminiclostridium thermocellum. It is an endo-1,4-beta-glucanase. The recombinant RtCel9K, purified from Escherichia coli, is a modular Glycoside Hydrolase family 9 (GH9) enzyme, with an N-terminal family 4 and family 30 Carbohydrate Binding Modules (CBM4 and CBM30, respectively) and a C-terminal dockerin (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Cellulases. Family: GH9. Ec Number: 3.2.1.4. Organism: Ruminiclostridium thermocellum. Architecture: CBM4-CBM30-GH9-doc. Optimal pH: 6. Optimal Temperature: 65°C. Substrates: avicel, cellulosic carbohydrates