Cellulase 5B & Mannanse 5A, Ruminococcus flavefaciens

Cellulase 5B & Mannanse 5A (RfCel5B-Man5A), assigned the E.C. number 3.2.1.4, is a derivative of Ruminococcus flavefaciens. It is an endo-1,4-beta-glucanase. The recombinant RfCel5B-Man5A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 5 (GH5) enzyme, with two GH5 catlytic module repeats and an internal family 80 Carbohydrate Binding Module (CBM80) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Cellulases & beta-Mannanases. Family: GH5. Ec Number: 3.2.1.4. Organism: Ruminococcus flavefaciens. Architecture: GH5-CBM80-GH5. Optimal pH: 7. Optimal Temperature: 37°C. Substrates: 1,3-1,4-beta-glucans, 1,4-beta-glucans, galactomannans, glucomannan, mannans, xyloglucan