Cell adhesion molecule 1 (Cadm1), partial (Active), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Mammalian cell. Expression Region: 48-388aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: QNLFTKDVTV IEGEVATISC QVNKSDDSVI QLLNPNRQTI YFRDFRPLKD SRFQLLNFSS SELKVSLTNV SISDEGRYFC QLYTDPPQES YTTITVLVPP RNLMIDIQKD TAVEGEEIEV NCTAMASKPA TTIRWFKGNK ELKGKSEVEE WSDMYTVTSQ LMLKVHKEDD GVPVICQVEH PAVTGNLQTQ RYLEVQYKPQ VHIQMTYPLQ GLTREGDAFE LTCEAIGKPQ PVMVTWVRVD DEMPQHAVLS GPNLFINNLN KTDNGTYRCE ASNIVGKAHS DYMLYVYDPP TTIPPPTTTT TTTTTTTTTI LTIITDTTAT TEPAVHDSRA GEEGTIGAVD H. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse CADM1 at 2 µg/mL can bind Mouse CRTAM (CSB-MP614820MO), the EC50 is 52.61-92.92 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mediates homophilic cell-cell adhesion. Reference: Biederer T., Sara Y., Mozhayeva M., Atasoy D., Liu X., Kavalali E.T., Sudhof T.C. Science 297:1525-1531(2002). Function: nan