CD24, Recombinant, Mouse:Fc (M1/69-J11D Heat Stable Antigen, Nectadrin, Lymphocyte Antigen 52, X62 H

CD24 is a highly glycosylated protein expressed as glycosyl-phosphatidyl-inositol (GPI) anchored molecule. Widely expressed, mainly on T cells, B cells and is upregulated in numerous types of cancers. It has costimulatory activity for antigen specific T cell responses. Polymorphisms of human CD24 are associated with several autoimmune diseases. Recently a new role as negative regulator of the immune response has been reported. CD24-deficient mice are more sensitive to inflammatory responses. CD24 together with the membrane protein SIGLEC interacts with danger-associated molecular patterns (DAMPs), such as HMGB1, HSP90 or HSP70. CD24/SIGLEC complex may differentially regulate the host response to DAMPs and PAMPs (pathogen-associated molecular patterns). Sequence: The extracellular domain of mouse CD24 (aa27-53) is fused at the C-terminus to the Fc portion of human IgG1, expressed in HEK293 cells. Molecular Weight: ~40-50kD (SDS-PAGE), Endotoxin: <0.06EU/ug (LAL test, Lonza). Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.