Cathepsin O (CTSO), human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 108-321aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-tagged. Target Protein Sequence: LPLRFDWRDK QVVTQVRNQQ MCGGCWAFSV VGAVESAYAI KGKPLEDLSV QQVIDCSYNN YGCNGGSTLN ALNWLNKMQV KLVKDSEYPF KAQNGLCHYF SGSHSGFSIK GYSAYDFSDQ EDEMAKALLT FGPLVVIVDA VSWQDYLGGI IQHHCSSGEA NHAVLITGFD KTGSTPYWIV RNSWGSSWGV DGYAHVKMGS NVCGIADSVS SIFV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Proteolytic enzyme possibly involved in normal cellular protein degradation and turnover. Reference: "First genome-wide association scan on neurophysiological endophenotypes points to trans-regulation effects on SLC2A3 in dyslexic children." Roeske D., Ludwig K.U., Neuhoff N., Becker J., Bartling J., Bruder J., Brockschmidt F.F., Warnke A., Remschmidt H., Hoffmann P., Muller-Myhsok B., Nothen M.M., Schulte-Korne G. Mol. Psychiatry 16:97-107(2011). Function: Proteolytic enzyme possibly involved in normal cellular protein degradation and turnover.