Cartilage intermediate layer protein 1 (CILP), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Baculovirus. Expression Region: 725-1184aa. Protein Length: Partial. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-tagged. Target Protein Sequence: EDRTFLVGNL EIRERRLFNL DVPESRRCFV KVRAYRSERF LPSEQIQGVV ISVINLEPRT GFLSNPRAWG RFDSVITGPN GACVPAFCDD QSPDAYSAYV LASLAGEELQ AVESSPKFNP NAIGVPQPYL NKLNYRRTDH EDPRVKKTAF QISMAKPRPN SAEESNGPIY AFENLRACEE APPSAAHFRF YQIEGDRYDY NTVPFNEDDP MSWTEDYLAW WPKPMEFRAC YIKVKIVGPL EVNVRSRNMG GTHRQTVGKL YGIRDVRSTR DRDQPNVSAA CLEFKCSGML YDQDRVDRTL VKVIPQGSCR RASVNPMLHE YLVNHLPLAV NNDTSEYTML APLDPLGHNY GIYTVTDQDP RTAKEIALGR CFDGTSDGSS RIMKSNVGVA LTFNCVERQV GRQSAFQYLQ STPAQSPAAG TVQGRVPSRR QQRASRGGQR QGGVVASLRF PRVAQQPLIN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage. Reference: "Genomic organization, mapping, and polymorphisms of the gene encoding human cartilage intermediate layer protein (CILP)." Nakamura I., Okawa A., Ikegawa S., Takaoka K., Nakamura Y. J. Hum. Genet. 44:203-205(1999). Function: Probably plays a role in cartilage scaffolding. May act by antagonizing TGF-beta1 (TGFB1) and IGF1 functions. Has the ability to suppress IGF1-induced proliferation and sulfated proteoglycan synthesis, and inhibits ligand-induced IGF1R autophosphorylation. May inhibit TGFB1-mediated induction of cartilage matrix genes via its interaction with TGFB1. Overexpression may lead to impair chondrocyte growth and matrix repair and indirectly promote inorganic pyrophosphate (PPi) supersaturation in aging and osteoarthritis cartilage.