Carnitine O-palmitoyltransferase 2, mitochondrial (CPT2),partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 209-658aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AYPLDMSQYF RLFNSTRLPK PSRDELFTDD KARHLLVLRK GNFYIFDVLD QDGNIVSPSE IQAHLKYILS DSSPAPEFPL AYLTSENRDI WAELRQKLMS SGNEESLRKV DSAVFCLCLD DFPIKDLVHL SHNMLHGDGT NRWFDKSFNL IIAKDGSTAV HFEHSWGDGV AVLRFFNEVF KDSTQTPAVT PQSQPATTDS TVTVQKLNFE LTDALKTGIT AAKEKFDATM KTLTIDCVQF QRGGKEFLKK QKLSPDAVAQ LAFQMAFLRQ YGQTVATYES CSTAAFKHGR TETIRPASVY TKRCSEAFVR EPSRHSAGEL QQMMVECSKY HGQLTKEAAM GQGFDRHLFA LRHLAAAKGI ILPELYLDPA YGQINHNVLS TSTLSSPAVN LGGFAPVVSD GFGVGYAVHD NWIGCNVSSY PGRNAREFLQ CVEKALEDMF DALEGKSIKS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites . Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters . Reference: "Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency." Yasuno T., Osafune K., Sakurai H., Asaka I., Tanaka A., Yamaguchi S., Yamada K., Hitomi H., Arai S., Kurose Y., Higaki Y., Sudo M., Ando S., Nakashima H., Saito T., Kaneoka H. Biochem. Biophys. Res. Commun. 448:175-181(2014). Function: nan