Cardiotrophin-1 (human, recombinant)

Cardiotrophin-1 is a glycosylated polypeptide and member of the IL-6 family of cytokines. It is composed of four alpha-helices but lacks the hydrophobic N-terminal secretion signal sequence that most IL-6 family proteins contain. Cardiotrophin-1 is primarily expressed in embryonic and adult cardiomyocytes, but it is also expressed in skeletal muscle, as well as the liver, lungs, and kidneys. Its expression is increased under cellular stress conditions, such as hypoxic stress. Cardiotrophin-1 forms a heterocomplex with gp130 and leukemia inhibitory factor receptor beta (LIFRbeta) in cardiomyocytes and, in other cells, with gp130, LIFRbeta, and cytokine-specific alpha-chains, which signal through the JAK/STAT, ERK/MAPK, and PI3K/AKT pathways. It induces cardiomyocyte hypertrophy in vitro, and levels of cardiotrophin-1 are increased in the heart in animal models after myocardial infarction and protect the heart from ischemic injury. Serum levels of cardiotrophin-1 are increased in patients with heart failure and hypertensive disease. It has both protective or detrimental effects in various tissues, including the heart, kidney, and vasculature. Cayman's Cardiotrophin-1 (human, recombinant) protein is a disulfide-linked homodimer. The reduced monomer, composed of cardiotrophin-1 (amino acids 2-201) fused to human IgG1 at its N-terminus, consists of 460 amino acids and has a calculated molecular weight of 49.5 kDa. As a result of glycosylation, the monomer migrates at approximately 54 kDa by SDS-PAGE under reducing conditions.Synonyms: CT-1, CT1, CTF1. Purity: >80% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Active recombinant N-terminal human IgG1 Fc-tagged cardiotrophin-1 expressed in HEK293 cells. Amino Acids: 2-201. MW: 49.5 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. UniProt Accession #: Q16619.