Carboxypeptidase D (CPD), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 383-461aa. Protein Length: Partial. Tag Info: C-terminal hFc-Myc-tagged. Target Protein Sequence: GVKGFVKDSI TGSGLENATI SVAGINHNIT TGRFGDFYRL LVPGTYNLTV VLTGYMPLTV TNVVVKEGPA TEVDFSLRP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalytic activity Releases C-terminal Arg and Lys from polypeptides. EC:3.4.17.22. Reference: "Sequence of human carboxypeptidase D reveals it to be a member of the regulatory carboxypeptidase family with three tandem active site domains." Tan F., Rehli M., Krause S.W., Skidgel R.A. Biochem. J. 327:81-87(1997). Function: nan