Caprin-1 (Caprin1), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 2-707aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: PSATSHSGSG SKSSGPPPPS GSSGSEAAAG AAAPASQHPA TGTGAVQTEA MKQILGVIDK KLRNLEKKKG KLDDYQERMN KGERLNQDQL DAVSKYQEVT NNLEFAKELQ RSFMALSQDI QKTIKKTARR EQLMREEAEQ KRLKTVLELQ YVLDKLGDDD VRTDLKQGLS GVPILSEEEL SLLDEFYKLV DPERDMSLRL NEQYEHASIH LWDLLEGKEK PVCGTTYKAL KEIVERVFQS NYFDSTHNHQ NGLCEEEEAA SAPIVEDQVA EAEPEPTEEY TEQSEVESTE YVNRQFMAET QFSSGEKEQV DEWAVETVEV VNSLQQQPQA ASPSVPEPHS LTPVAQSDPL VRRQRVQDLM AQMQGPYNFI QDSMLDFENQ TLDPAIVSAQ PMNPTQNMDM PQLVCPQVHS ESRLAQSNQV PVQPEATQVP LVSSTSEGYT ASQPLYQPSH AAEQRPQKEP IDQIQATISL NTEQTTASSS LPAASQPQVF QAGASKPLHS SGINVNAAPF QSMQTVFNMN APVPPVNEPE TLKQQSQYQA SYNQSFSSQP HQVEQTELQQ DQLQTVVGTY HGSQDQPHQV PGNHQQPPQQ STGFPRSSQP YYNSRGVSRG GSRGARGLMN GYRGPANGFR GGYDGYRSSF SNTPNSGYTQ SQFNAPRDYS GYQRDGYQQN FKRGSGQSGP RGAPRGRGGP PRPNRGMPQM NTQQVN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Reference: A novel RNA-binding protein in neuronal RNA granules regulatory machinery for local translation.Shiina N., Shinkura K., Tokunaga M.J. Neurosci. 25:4420-4434(2005). Function: May regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Binds directly and selectively to MYC and CCND2 RNAs. In neuronal cells, directly binds to several mRNAs associated with RNA granules, including BDNF, CAMK2A, CREB1, MAP2, NTRK2 mRNAs, as well as to GRIN1 and KPNB1 mRNAs, but not to rRNAs.