Calpain-3 (CAPN3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 74-417aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: LYVDPEFPPD ETSLFYSQKF PIQFVWKRPP EICENPRFII DGANRTDICQ GELGDCWFLA AIACLTLNQH LLFRVIPHDQ SFIENYAGIF HFQFWRYGEW VDVVIDDCLP TYNNQLVFTK SNHRNEFWSA LLEKAYAKLH GSYEALKGGN TTEAMEDFTG GVAEFFEIRD APSDMYKIMK KAIERGSLMG CSIDDGTNMT YGTSPSGLNM GELIARMVRN MDNSLLQDSD LDPRGSDERP TRTIIPVQYE TRMACGLVRG HAYSVTGLDE VPFKGEKVKL VRLRNPWGQV EWNGSWSDRW KDWSFVDKDE KARLQHQVTE DGEFWMSYED FIYHFTKLEI CNLT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: n/a. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1 at 'His-409'. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53. Reference: n/a. Function: nan