Calmodulin-domain protein kinase 1 (CDPK1), Toxoplasma gondii, recombinant

Organism: Toxoplasma gondii. Source: E.coli. Expression Region: 1-507aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MGQQESTLGG AAGEPRSRGH AAGTSGGPGD HLHATPGMFV QHSTAIFSDR YKGQRVLGKG SFGEVILCKD KITGQECAVK VISKRQVKQK TDKESLLREV QLLKQLDHPN IMKLYEFFED KGYFYLVGEV YTGGELFDEI ISRKRFSEVD AARIIRQVLS GITYMHKNKI VHRDLKPENL LLESKSKDAN IRIIDFGLST HFEASKKMKD KIGTAYYIAP EVLHGTYDEK CDVWSTGVIL YILLSGCPPF NGANEYDILK KVEKGKYTFE LPQWKKVSES AKDLIRKMLT YVPSMRISAR DALDHEWIQT YTKEQISVDV PSLDNAILNI RQFQGTQKLA QAALLYMGSK LTSQDETKEL TAIFHKMDKN GDGQLDRAEL IEGYKELMRM KGQDASMLDA SAVEHEVDQV LDAVDFDKNG YIEYSEFVTV AMDRKTLLSR ERLERAFRMF DSDNSGKISS TELATIFGVS DVDSETWKSV LSEVDKNNDG EVDFDEFQQM LLKLCGN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Discovery of Potent and Selective Inhibitors of Calcium-Dependent Protein Kinase 1 (CDPK1) from C. parvum and T. gondii." Murphy R.C., Ojo K.K., Larson E.T., Castellanos-Gonzalez A., Perera B.G., Keyloun K.R., Kim J.E., Bhandari J.G., Muller N.R., Verlinde C.L., White A.C., Merritt E.A., Van Voorhis W.C., Maly D.J. ACS Med. Chem. Lett. 1:331-335(2010). Function: nan