Cadherin-20 (CDH20), partial, recombinant human

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 60-619aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: SWVWNQFFVL EEYTGTDPLY VGKLHSDMDR GDGSIKYILS GEGAGIVFTI DDTTGDIHAI QRLDREERAQ YTLRAQALDR RTGRPMEPES EFIIKIQDIN DNEPKFLDGP YVATVPEMSP VGTSVIQVTA TDADDPTYGN SARVVYSILQ GQPYFSVDSK TGVIRTALMN MDREAKEYYE VIIQAKDMGG QLGGLAGTTT VNITLSDVND NPPRFPQKHY QMSVLESAPI SSTVGRVFAK DLDEGINAEM KYTIVDGDGA DAFDISTDPN FQVGIITVKK PLSFESKKSY TLKVEGANPH LEMRFLNLGP FQDTTTVHIS VEDVDEPPVF EPGFYFVEVP EDVAIGTTIQ IISAKDPDVT NNSIRYSIDR SSDPGRFFYV DITTGALMTA RPLDREEFSW HNITVLAMEM NNPSQVGSVP VTIKVLDVND NAPEFPRFYE AFVCENAKAG QLIQTVSAVD QDDPRNGQHF YYSLAPEAAN NPNFTIRDNQ DNTARILTRR SGFRQQEQSV FHLPILIADS GQPVLSSTGT LTIQVCSCDD DGHVMSCSPE AYMLPVSLSR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: n/a. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Reference: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose J.E., Behm F.M., Drgon T., Johnson C., Uhl G.R. Mol Med 16:247-253 (2010). Function: nan