C5a peptidase (scpA), partial, recombinant Streptococcus pyogenes

Organism: Streptococcus pyogenes. Source: E.coli. Expression Region: 96-584aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: ATSKATIRDL NDPSQVKTLQ EKAGKGAGTV VAVIDAGFDK NHEAWRLTDK TKARYQSKED LEKAKKEHGI TYGEWVNDKV AYYHDYSKDG KTAVDQEHGT HVSGILSGNA PSETKEPYRL EGAMPEAQLL LMRVEIVNGL ADYARNYAQA IRDAVNLGAK VINMSFGNAA LAYANLPDET KKAFDYAKSK GVSIVTSAGN DSSFGGKTRL PLADHPDYGV VGTPAAADST LTVASYSPDK QLTETAMVKT DDQQDKEMPV LSTNRFEPNK AYDYAYANRG MKEDDFKDVK GKIALIERGD IDFKDKVANA KKAGAVGVLI YDNQDKGFPI ELPNVDQMPA AFISRKDGLL LKDNPQKTIT FNATPKVLPT ASGTKLSRFS SWGLTADGNI KPDIAAPGQD ILSSVANNKY AKLSGTSMSA PLVAGIMGLL QKQYETQYPD MTPSERLDLA KKVLMSSATA LYDEDEKAYF SPRQQGAGAV DAKKASAAT. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: Not Test. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: This virulence factor of S.pyogenes specifically cleaves the human serum chemotaxin C5a at '68-Lys-, -Asp-69' bond near its C-terminus, destroying its ability to serve as a chemoattractant. Reference: n/a. Function: nan