Butyrophilin-like protein 3 (BTNL3), partial, Biotinylated, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 18-237aa. Protein Length: Partial. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: QWQVTGPGKF VQALVGEDAV FSCSLFPETS AEAMEVRFFR NQFHAVVHLY RDGEDWESKQ MPQYRGRTEF VKDSIAGGRV SLRLKNITPS DIGLYGCWFS SQIYDEEATW ELRVAALGSL PLISIVGYVD GGIQLLCLSS GWFPQPTAKW KGPQGQDLSS DSRANADGYS LYDVEISIIV QENAGSILCS IHLAEQSHEV ESKVLIGETF FQPSPWRLAS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Alpha-(1,4) exo-glucosidase involved in breakdown of dietary starch oligosaccharides in small intestine. Cleaves the non-reducing alpha-(1,4)-linked glucose residue in linear dextrins with retention of anomeric center stereochemistry . Mainly hydrolyzes short length oligomaltoses having two to seven glucose residues . Can cleave alpha-(1,2), alpha-(1,3) and alpha-(1,6) glycosidic linkages with lower efficiency, whereas beta glycosidic linkages are usually not hydrolyzed . Reference: "Contribution of the Individual Small Intestinal alpha-Glucosidases to Digestion of Unusual alpha-Linked Glycemic Disaccharides." Lee B.H., Rose D.R., Lin A.H., Quezada-Calvillo R., Nichols B.L., Hamaker B.R. J. Agric. Food Chem. 64:6487-6494(2016). Function: nan