BMX, Active, Recombinant, Human, aa1-675, GST-tag (ETK, PSCTK2, PSCTK3, Bone Marrow Tyrosine Kinase

The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells. The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX, is found to induce activation of the Stat signaling pathway. Source: Recombinant protein corresponding to aa1-675 from full-length human cytoplasmic tyrosine-protein kinase BMX, fused to GST-tag at N-terminal, expressed in Sf9 insect cells. Molecular Weight: ~110kD, Specific Activity: ~43pmol/min/ug, Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Assay Conditions: Assay was done in Kinase buffer containing 0.2mM DTT using Poly-(Tyr,Glu) substrate (0.2mg/ml) and 20mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was measured using Kinase- Glo reagent. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.