Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1 (NDST1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 40-556aa. Protein Length: Partial of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: GWKRGLEPSA DAPEPDCGDP PPVAPSRLLP LKPVQAATPS RTDPLVLVFV ESLYSQLGQE VVAILESSRF KYRTEIAPGK GDMPTLTDKG RGRFALIIYE NILKYVNLDA WNRELLDKYC VAYGVGIIGF FKANENSLLS AQLKGFPLFL HSNLGLKDCS INPKSPLLYV TRPSEVEKGV LPGEDWTVFQ SNHSTYEPVL LAKTRSSESI PHLGADAGLH AALHATVVQD LGLHDGIQRV LFGNNLNFWL HKLVFVDAVA FLTGKRLSLP LDRYILVDID DIFVGKEGTR MKVEDVKALF DTQNELRAHI PNFTFNLGYS GKFFHTGTNA EDAGDDLLLS YVKEFWWFPH MWSHMQPHLF HNQSVLAEQM ALNKKFAVEH GIPTDMGYAV APHHSGVYPV HVQLYEAWKQ VWSIRVTSTE EYPHLKPARY RRGFIHNGIM VLPRQTCGLF THTIFYNEYP GGSSELDKII NGGELFLTVL LNPVSAPQPM AAGEKGLLHS LSAADTGFLE PGKGGEA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. Reference: NDST1 missense mutations in autosomal recessive intellectual disability.Reuter M.S., Musante L., Hu H., Diederich S., Sticht H., Ekici A.B., Uebe S., Wienker T.F., Bartsch O., Zechner U., Oppitz C., Keleman K., Jamra R.A., Najmabadi H., Schweiger S., Reis A., Kahrizi K.Am. J. Med. Genet. A 164A:2753-2763(2014). Function: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis