Beta-amylase 3, chloroplastic (BAM3), Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: E.coli. Expression Region: 50-548aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: EMKFTHEKTF TPEGETLEKW EKLHVLSYPH SKNDASVPVF VMLPLDTVTM SGHLNKPRAM NASLMALKGA GVEGVMVDAW WGLVEKDGPM NYNWEGYAEL IQMVQKHGLK LQVVMSFHQC GGNVGDSCSI PLPPWVLEEI SKNPDLVYTD KSGRRNPEYI SLGCDSVPVL RGRTPIQVYS DFMRSFRERF EGYIGGVIAE IQVGMGPCGE LRYPSYPESN GTWRFPGIGE FQCYDKYMKS SLQAYAESIG KTNWGTSGPH DAGEYKNLPE DTEFFRRDGT WNSEYGKFFM EWYSGKLLEH GDQLLSSAKG IFQGSGAKLS GKVAGIHWHY NTRSHAAELT AGYYNTRNHD GYLPIAKMFN KHGVVLNFTC MEMKDGEQPE HANCSPEGLV KQVQNATRQA GTELAGENAL ERYDSSAFGQ VVATNRSDSG NGLTAFTYLR MNKRLFEGQN WQQLVEFVKN MKEGGHGRRL SKEDTTGSDL YVGFVKGKIA ENVEEAALV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Beta-amylase activity. No alpha-amylase activity. Involved in cold resistance. Mediates the accumulation of maltose upon freezing stress, thus contributing to the protection of the photosynthetic electron transport chain. Plays a role in the circadian-regulated starch degradation and maltose metabolism in chloroplasts, especially at night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan. Reference: "Araport11: a complete reannotation of the Arabidopsis thaliana reference genome." Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., Town C.D. Plant J. 89:789-804(2017). Function: nan