Beta-amylase 1, chloroplastic (BAM1),partial, Arabidopsis thaliana, recombinant

Organism: Arabidopsis thaliana (Mouse-ear cress). Source: E.coli. Expression Region: 42-354aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: AMNRNYKAHG TDPSPPMSPI LGATRADLSV ACKAFAVENG IGTIEEQRTY REGGIGGKKE GGGGVPVFVM MPLDSVTMGN TVNRRKAMKA SLQALKSAGV EGIMIDVWWG LVEKESPGTY NWGGYNELLE LAKKLGLKVQ AVMSFHQCGG NVGDSVTIPL PQWVVEEVDK DPDLAYTDQW GRRNHEYISL GADTLPVLKG RTPVQCYADF MRAFRDNFKH LLGETIVEIQ VGMGPAGELR YPSYPEQEGT WKFPGIGAFQ CYDKYSLSSL KAAAETYGKP EWGSTGPTDA GHYNNWPEDT QFFKKEGGGW NSE. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Beta-amylase activity. Can use p-nitrophenyl maltopentaoside as substrate only in reduced form. Can play a minor role in the starch degradation and maltose metabolism in chloroplasts during the night. More active on phosphorylated glucan. Interacts directly with starch or other alpha-1,4-glucan. Reference: "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003). Function: nan