beta-Acetylglucosaminidase 85A, Streptococcus pneumoniae

beta-Acetylglucosaminidase 85A (SpAcp85A), assigned the E.C. number 3.2.1.96, is a derivative of Streptococcus pneumoniae. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant SpAcp85A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 85 (GH85) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Glycoside Hydrolase. Activity: Acetylglucosaminidases. Family: GH85. Ec Number: 3.2.1.96. Organism: Streptococcus pneumoniae. Architecture: GH85. Optimal pH: 7.5. Optimal Temperature: 37°C. Substrates: 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside (3F4NP-GlcNAc), chitobiose core of N-glycans