Basement membrane-specific heparan sulfate proteoglycan core protein (HSPG2), partial, recombinant h

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 4197-4391aa. Protein Length: Partial. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: DAPGQYGAYF HDDGFLAFPG HVFSRSLPEV PETIELEVRT STASGLLLWQ GVEVGEAGQG KDFISLGLQD GHLVFRYQLG SGEARLVSED PINDGEWHRV TALREGRRGS IQVDGEELVS GRSPGPNVAV NAKGSVYIGG APDVATLTGG RFSSGITGCV KNLVLHSARP GAPPPQPLDL QHRAQAGANT RPCPS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: Integral component of basement membranes. Component of the glomerular basement membrane , responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Reference: n/a. Function: nan