B-XpanderTM CD40L (human) (rec.) (Animal Free)

B-Xpander(TM) is a potent enhanced Multimeric human CD40L cytokine, for B cell activation, B cell expansion (proliferation) and cell therapy applications. In-house production using a proprietary protocol and a characterized and certified CHO cell line allows the supply of the most active Multimeric human CD40L proteins in GMP-like format. Interest in B cells has recently increased in immunotherapies using Engineered B cells or Tumor-Infiltrating Lymphocytes (TILs). However, current methods for B cell expansion are inefficient, time consuming and can lead to insufficient B cell numbers and persistent T cell exhaustion. Our B-Xpander(TM) protein improves current protocols for B cell stimulation by mimicking the natural interaction between CD40L and CD40 molecules on B cells. B-XpanderTM Quality Features:  Production in characterized and certified CHO cell line (for GMP-grade manufacturing)*  High Bioactivity tested by ELISA/Cell-based Assays  Verified Purity & Homogeneity by SEC  Low Endotoxin Level  Batch-to-Batch Consistency  Animal-component free Production  No cross-linking Reagents necessary. Source/Host: CHO cells. Produced using animal component-free medium. Sequence: Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG(R)-tag. Crossreactivity: Human. Application: Can be used for B cell expansion or during TIL expansion to increase the activation of T cells. Specificity: Binds to human CD40. Biological Activity: Induces B cell activation and B cell expansion (concentrations depend on the protocol, but we recommend to start at 50-100 ng/ml). Endotoxin Content: <0.01EU/µg purified protein (LAL test, Lonza).