B-cell scaffold protein with ankyrin repeats (BANK1), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 1-785aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MLPAAPGKGL GSPDPAPCGP APPGNTKDII MIYEEDAEEW ALYLTEVFLH VVKREAILLY RLENFSFRHL ELLNLTSYKC KLLILSNSLL RDLTPKKCQF LEKILHSPKS VVTLLCGVKS SDQLYELLNI SQSRWEISTE QEPEDYISVI QSIIFKDSED YFEVNIPTDL RAKHSGEISE RKEIEELSEA SRNTIPLAVV LPTEIPCENP GEIFIILRDE VIGDTVEVEF TSSNKRIRTR PALWNKKVWC MKALEFPAGS VHVNVYCDGI VKATTKIKYY PTAKAKECLF RMADSGESLC QNSIEELDGV LTSIFKHEIP YYEFQSLQTE ICSQNKYTHF KELPTLLHCA AKFGLKNLAI HLLQCSGATW ASKMKNMEGS DPAHIAERHG HKELKKIFED FSIQEIDINN EQENDYEEDI ASFSTYIPST QNPAFHHESR KTYGQSADGA EANEMEGEGK QNGSGMETKH SPLEVGSESS EDQYDDLYVF IPGADPENNS QEPLMSSRPP LPPPRPVANA FQLERPHFTL PGTMVEGQME RSQNWGHPGV RQETGDEPKG EKEKKEEEKE QEEEEDPYTF AEIDDSEYDM ILANLSIKKK TGSRSFIINR PPAPTPRPTS IPPKEETTPY IAQVFQQKTA RRQSDDDKFC GLPKKQDRAR IESPAFSTLR GCLTDGQEEL ILLQEKVKNG KMSMDEALEK FKHWQMGKSG LEMIQQEKLR QLRDCIIGKR PEEENVYNKL TIVHHPGGKE TAHNENKFYN VHFSNKLPAR PQVEKEFGFC CKKDH. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in B-cell receptor (BCR)-induced Ca2+ mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2. Reference: BANK regulates BCR-induced calcium mobilization by promoting tyrosine phosphorylation of IP3 receptor.Yokoyama K., Su I., Tezuka T., Yasuda T., Mikoshiba K., Tarakhovsky A., Yamamoto T.EMBO J. 21:83-92(2002). Function: Involved in B-cell receptor (BCR)-induced Ca(2+) mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2.