Autolysin (lytA), Staphylococcus aureus, recombinant

Organism: Staphylococcus aureus. Source: E.coli. Expression Region: 1-481aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MQAKLTKNEF IERLKTSEGK QFNVDLWYGF QCFDYANAGW KVLFGLLLKG LGAKDIPFAN NFDGLATVYQ NTPDFLAQPG DMVVFGSNYG AGYGHVAWVI EATLDYIIVY EQNWLGGGWT DGIEQPAGVG KKLQDDNMLM ISLCGLSVRI LKVRQRHDQF NLLHKHPKKE TAKPQPKAVE LKIIKDVVKG YDLPKRGSNP KGIVIHNDAG SKGATAEAYR NGLVNAPLSR LEAGIAHSYV SGNTVWQALD ESQVGWHTAN QIGNKYYYGI EVCQSMGADN ATFLKNEQAT FQECARLLKK WGLPANRNTI RLHNEFTSTS CPHRSSVLHT GFDPVTRGLL PEDKRLQLKD YFIKQIRAYM DGKIPVATVS NESSASSNTV KPVASAWKRN KYGTYYMEES ARFTNGNQPI TVRKVGPFLS CPVGYQFQPG GYCDYTEVML QDGHVWVGYT WEGQRYYLPI RTWNGSAPPN QILGDLWGEI S. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity. Reference: "Sequence analysis of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity." Wang X., Wilkinson B.J., Jayaswal R.K. Gene 102:105-109(1991). Function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Autolysin strictly depends on the presence of choline-containing cell walls for activity.