ATP synthase subunit alpha, mitochondrial (ATP5F1A), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 44-553aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: QKTGTAEMSS ILEERILGAD TSVDLEETGR VLSIGDGIAR VHGLRNVQAE EMVEFSSGLK GMSLNLEPDN VGVVVFGNDK LIKEGDIVKR TGAIVDVPVG EELLGRVVDA LGNAIDGKGP IGSKTRRRVG LKAPGIIPRI SVREPMQTGI KAVDSLVPIG RGQRELIIGD RQTGKTSIAI DTIINQKRFN DGSDEKKKLY CIYVAIGQKR STVAQLVKRL TDADAMKYTI VVSATASDAA PLQYLAPYSG CSMGEYFRDN GKHALIIYDD LSKQAVAYRQ MSLLLRRPPG REAYPGDVFY LHSRLLERAA KMNDAFGGGS LTALPVIETQ AGDVSAYIPT NVISITDGQI FLETELFYKG IRPAINVGLS VSRVGSAAQT RAMKQVAGTM KLELAQYREV AAFAQFGSDL DAATQQLLSR GVRLTELLKQ GQYSPMAIEE QVAVIYAGVR GYLDKLEPSK ITKFENAFLS HVVSQHQALL GTIRADGKIS EQSDAKLKEI VTNFLAGFEA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Reference: Nucleotide sequence of a cDNA for the alpha subunit of human mitochondrial ATP synthase.Kataoka H., Biswas C.Biochim. Biophys. Acta 1089:393-395(1991). Function: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites (By similarity).