Asporin (Aspn), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 33-373aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: DMEDTDDDDN DDDDNSLFPT KEPVNPFFPF DLFPTCPFGC QCYSRVVHCS DLGLTSVPNN IPFDTRMVDL QNNKIKEIKE NDFKGLTSLY ALILNNNKLT KIHPKTFLTT KKLRRLYLSH NQLSEIPLNL PKSLAELRIH DNKVKKIQKD TFKGMNALHV LEMSANPLEN NGIEPGAFEG VTVFHIRIAE AKLTSIPKGL PPTLLELHLD FNKISTVELE DLKRYRELQR LGLGNNRITD IENGTFANIP RVREIHLEHN KLKKIPSGLQ ELKYLQIIFL HYNSIAKVGV NDFCPTVPKM KKSLYSAISL FNNPMKYWEI QPATFRCVLG RMSVQLGNVG K. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity. Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins. Inhibits the interaction between TGFB1 and TGF-beta receptor type II in the presence of heparin/heparan sulfate in vitro. Reference: "Binding characteristics of the osteoarthritis-associated protein asporin." Kou I., Nakajima M., Ikegawa S. J. Bone Miner. Metab. 28:395-402(2010). Function: nan