Arginase-2, mitochondrial (Arg2), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 23-354aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: VHSVAIVGAP FSRGQKKLGV EYGPAAIREA GLLKRLSRLG CHLKDFGDLS FTNVPQDDPY NNLVVYPRSV GLANQELAEV VSRAVSGGYS CVTMGGDHSL AIGTIIGHAR HRPDLCVIWV DAHADINTPL TTVSGNIHGQ PLSFLIKELQ DKVPQLPGFS WIKPCLSPPN IVYIGLRDVE PPEHFILKNY DIQYFSMREI DRLGIQKVME QTFDRLIGKR QRPIHLSFDI DAFDPKLAPA TGTPVVGGLT YREGVYITEE IHNTGLLSAL DLVEVNPHLA TSEEEAKATA RLAVDVIASS FGQTREGGHI VYDHLPTPSS PHESENEECV RI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Reference: Cloning and characterization of the mouse and rat type II arginase genes.Iyer R.K., Bando J.M., Jenkinson C.P., Vockley J.G., Kim P.S., Kern R.M., Cederbaum S.D., Grody W.W.Mol. Genet. Metab. 63:168-175(1998). Function: May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to nitric oxid synthase (NOS). Arginine metabolism is a critical regulator of innate and adaptive immune responses. Seems to be involved in negative regulation of the survival capacity of activated CD4(+) and CD8(+) T cells