Anti-Perfringolysin O (C459A mutant, C. perfringens, recombinant)

Perfringolysin O (PFO) is a protein member of the cholesterol-dependent cytolysin (CDC) family. It is composed of four beta-sheet-rich domains and a C-terminal undecapeptide motif. PFO is a toxin secreted by C. perfringens and induces cholesterol-dependent cytolytic beta-barrel pore formation in the plasma membrane of host cells, such as erythrocytes and leukocytes. PFO containing a cysteine-to-alanine substitution at position 459 (PFOC459A) has a higher cholesterol membrane concentration threshold for pore formation compared to wild-type PFO and has been used as a cell membrane permeability agent in mitochondrial and metabolic studies. Cayman's Perfringolysin O (C459A mutant, C. perfringens, recombinant) protein can be used for cell-based assays.