Annexin V-mFluor(TM) Blue 510 conjugate

Ex: 505 nm. Em: 511 nm. Soluble in: Water. Annexins are a family of proteins that bind to phospholipid membranes in the presence of calcium. Annexin V is a valuable tool for studying cell apoptosis. It is used as a probe to detect cells which have expressed phosphatidylserine on the cell surface, a feature found in apoptosis as well as other forms of cell death. There are a variety of parameters that can be used for monitoring cell viability. Annexin V-dye conjugates are widely used to monitor cell apoptosis through measuring the translocation of phosphatidylserine (PS). In apoptosis, PS is transferred to the outer leaflet of the plasma membrane. The appearance of phosphatidylserine on the cell surface is a universal indicator of the initial/intermediate stages of cell apoptosis and can be detected before morphological changes can be observed. This Annexin V conjugate has the spectral properties Ex/Em = ~500/510 nm. The green fluorescent stain is well excited with the Blue Laser at 488 nm, and emits intense blue fluorescence at ~510 nm. Its emission is much sharper than those of Annexin V-FITC and Annexin V-Alexa Fluor 488 conjugates, thus requiring less compensation with multicolor flow cytometry.