Annexin A3 (ANXA3), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 5-322aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: WVGHRGTVRD YPDFSPSVDA EAIQKAIRGI GTDEKMLISI LTERSNAQRQ LIVKEYQAAY GKELKDDLKG DLSGHFEHLM VALVTPPAVF DAKQLKKSMK GAGTNEDALI EILTTRTSRQ MKDISQAYYT VYKKSLGDDI SSETSGDFRK ALLTLADGRR DESLKVDEHL AKQDAQILYK AGENRWGTDE DKFTEILCLR SFPQLKLTFD EYRNISQKDI VDSIKGELSG HFEDLLLAIV NCVRNTPAFL AERLHRALKG IGTDEFTLNR IMVSRSEIDL LDIRTEFKKH YGYSLYSAIK SDTSGDYEIT LLKICGGD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. Reference: Five distinct calcium and phospholipid binding proteins share homology with lipocortin I.Pepinsky R.B., Tizard R., Mattaliano R.J., Sinclair L.K., Miller G.T., Browning J.L., Chow E.P., Burne C., Huang K.-S., Pratt D., Wachter L., Hession C., Frey A.Z., Wallner B.P.J. Biol. Chem. 263:10799-10811(1988). Function: Inhibitor of phospholipase A2, also possesses anti-coagulant properties. Also cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate.