Angiotensin-converting enzyme 2 (ACE2), partial (Active), Paguma larvata, recombinant

Organism: Paguma larvata (Masked palm civet). Source: Mammalian cell. Expression Region: 18-740aa. Protein Length: Partial. Tag Info: C-terminal hFc-tagged. Target Protein Sequence: QSTTEELAKT FLETFNYEAQ ELSYQSSVAS WNYNTNITDE NAKNMNEAGA KWSAYYEEQS KLAQTYPLAE IQDAKIKRQL QALQQSGSSV LSADKSQRLN TILNAMSTIY STGKACNPNN PQECLLLEPG LDNIMENSKD YNERLWAWEG WRAEVGKQLR PLYEEYVALK NEMARANNYE DYGDYWRGDY EEEWTGGYNY SRNQLIQDVE DTFEQIKPLY QHLHAYVRAK LMDTYPSRIS RTGCLPAHLL GDMWGRFWTN LYPLTVPFGQ KPNIDVTDAM VNQNWDARRI FKEAEKFFVS VGLPNMTQGF WENSMLTEPG DGRKVVCHPT AWDLGKGDFR IKMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPNH LKTIGLLSPA FSEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGA IPKEQWMQKW WEMKRNIVGV VEPVPHDETY CDPASLFHVA NDYSFIRYYT RTIYQFQFQE ALCQIAKHEG PLHKCDISNS TEAGKKLLEM LSLGRSEPWT LALERVVGAK NMNVTPLLNY FEPLFTWLKE QNRNSFVGWD TDWRPYSDQS IKVRISLKSA LGEKAYEWND NEMYLFRSSI AYAMREYFSK VKNQTIPFVE DNVWVSDLKP RISFNFFVTF SNNVSDVIPR SEVEDAIRMS RSRINDAFRL DDNSLEFLGI EPTLSPPYRP PVT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: ?Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV S-RBD (CSB-MP348663HQE) at 2 µg/ml can bind Paguma larvata ACE2, the EC50 is 5.056-7.559 ng/ml.?Recombinant Paguma larvata ACE2 protein (CSB-MP684964PAL) enzyme activity is measured by its ability to cleave fluorogenic peptide substrate(Mca-Ala-Pro-Lys(Dnp)-OH), The Km is 22.84 µM. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered PBS, 6% Trehalose, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: n/a. Function: nan