Angiopoietin-1 (ANGPT1), Canis lupus familiaris, recombinant

Organism: Canis lupus familiaris (Dog) (Canis familiaris). Source: Mammalian cell. Expression Region: 20-497aa. Protein Length: Full Length of Mature Protein. Tag Info: C-terminal 6xHis-tagged. Target Protein Sequence: SNQRRSPENG GRRYNRIQHG QCAYTFILPE HDGNCRESTT DQYNTNALQR DAPHVGPDFS SQKLQHLEHV MENYTQWLQK IENYIVENMK SEMAQIQQNA VQNHTATMLE IGTSLLSQTA EQTRKLTDVE TQVLNQTSRL EIQLLENSLS TYKLEKQLLQ QTNEILKIHE KNSLLEHKIL EMEGKHKEEW DTLKEERENL QVLVTRQTYI IQELEKQLNR ATNNNSVLQK QQLELMDTVH NLVNLCTKEV LLKGGKKEEE KPFRDCADVY QAGFNKSGIY TIYINNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN PSGEYWLGNE FIFAITSQRQ YTLRIELMDC EGNRAYSQYD RFHIGNEKQN YRLYLKCHTG TAGKQSSLIL HGADFSTKDA DNDNCMCKCA LMLTGGWWFD ACGPSNLNGM FYTAGQNHGK LNGIKWHYFK GPSYSLRSTT MMIRPLDF. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development . Reference: "Gene expressions of canine angiopoietin-1 and -2 in normal tissues and spontaneous tumours." Kato Y., Asano K., Mizutani I., Konno T., Sasaki Y., Kutara K., Teshima K., Edamura K., Kano R., Suzuki K., Shibuya H., Sato T., Hasegawa A., Tanaka S. Res. Vet. Sci. 81:280-286(2006). Function: nan