Amphoterin-induced protein 3 (Amigo3), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: Mammalian cell. Expression Region: 20-383aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-Myc-tagged. Target Protein Sequence: TSDLEGVLPP DPHNCPNKCV CAADVLSCAG RGLQDLPAAL PATAAELDLS HNALKRLHPG WLAPLSRLRA LYLGYNKLDV LGRGVFTNAS GLRILDLSSN LLRRLRTYDL DGLEELEKLL LFNNRLMHLD LDAFQGLSML SHLYLSCNEL SSFSFNHLHG LGLTRLRTLD LSSNWLGHVS VPELAALPTF LKNRLYLHNN PLPCDCSLYH LLRRWHQRGL SALHDFEREY TCLAFKVAES RVRFFEHSRV FKNCSVAAAP GLELPEEELH THVGQSLRLF CNTTVPAARV AWVSPKNELL VAPGSQDGSI AVLADGSLAI GRVQEQHAGL FVCLASGPRL HHNQTLEYNV SVHKPRPEPE AFNT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May mediate heterophilic cell-cell interaction. May contribute to signal transduction through its intracellular domain. Reference: "AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats."Kuja-Panula J., Kiiltomaeki M., Yamashiro T., Rouhiainen A., Rauvala H.J. Cell Biol. 160:963-973(2003). Function: May mediate heterophilic cell-cell interaction. May contribute to signal transduction through its intracellular domain.