Alpha/beta-gliadin MM1, Triticum aestivum, recombinant

Organism: Triticum aestivum (Wheat). Source: E.coli. Expression Region: 21-307aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: VRVPVPQLQP QNPSQQQPQE QVPLVQQQQF PGQQQPFPPQ QPYPQPQPFP SQQPYLQLQP FPQPQLPYPQ PQLPYPQPQL PYPQPQPFRP QQPYPQSQPQ YSQPQQPISQ QQQQQQQQQQ QKQQQQQQQQ ILQQILQQQL IPCRDVVLQQ HSIAYGSSQV LQQSTYQLVQ QLCCQQLWQI PEQSRCQAIH NVVHAIILHQ QQQQQQQQQQ QPLSQVSFQQ PQQQYPSGQG SFQPSQQNPQ AQGSVQPQQL PQFEEIRNLA LETLPAMCNV YIPPYCTIAP VGIFGTN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Gliadin is the major seed storage protein in wheat. Reference: "A structural and immunological basis for the role of human leukocyte antigen DQ8 in celiac disease."Henderson K.N., Tye-Din J.A., Reid H.H., Chen Z., Borg N.A., Beissbarth T., Tatham A., Mannering S.I., Purcell A.W., Dudek N.L., van Heel D.A., McCluskey J., Rossjohn J., Anderson R.P.Immunity 27:23-34(2007). Function: Gliadin is the major seed storage protein in wheat.