Alpha-fetoprotein (Afp), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 19-605aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag-Free. Target Protein Sequence: KALHENEFGI ASTLDSSQCV TEKNVLSIAT ITFTQFVPEA TEEEVNKMTS DVLAAMKKNS GDGCLESQLS VFLDEICHET ELSNKYGLSG CCSQSGVERH QCLLARKKTA PASVPPFQFP EPAESCKAHE ENRAVFMNRF IYEVSRRNPF MYAPAILSLA AQYDKVVLAC CKADNKEECF QTKRASIAKE LREGSMLNEH VCSVIRKFGS RNLQATTIIK LSQKLTEANF TEIQKLALDV AHIHEECCQG NSLECLQDGE KVMTYICSQQ NILSSKIAEC CKLPMIQLGF CIIHAENGVK PEGLSLNPSQ FLGDRNFAQF SSEEKIMFMA SFLHEYSRTH PNLPVSVILR IAKTYQEILE KCSQSGNLPG CQDNLEEELQ KHIEESQALS KQSCALYQTL GDYKLQNLFL IGYTRKAPQL TSAELIDLTG KMVSIASTCC QLSEEKWSGC GEGMADIFIG HLCIRNEASP VNSGISHCCN SSYSNRRLCI TSFLRDETYA PPPFSEDKFI FHKDLCQAQG KALQTMKQEL LINLVKQKPE LTEEQLAAVT ADFSGLLEKC CKAQDQEVCF TEEGPKLISK TRDALGV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Binds estrogens, fatty acids and metals. Reference: "Homology between the primary structure of alpha-fetoprotein, deduced from a complete cDNA sequence, and serum albumin." Law S.W., Dugaiczyk A. Nature 291:201-205(1981). Function: Binds estrogens, fatty acids and metals.