Alpha-enolase (Eno1), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 2-433aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SILRIHAREI FDSRGNPTVE VDLYTAKGLF RAAVPSGAST GIYEALELRD NDKTRFMGKG VSQAVEHINK TIAPALVSKK VNVVEQEKID KLMIEMDGTE NKSKFGANAI LGVSLAVCKA GAVEKGVPLY RHIADLAGNP EVILPVPAFN VINGGSHAGN KLAMQEFMIL PVGASSFREA MRIGAEVYHN LKNVIKEKYG KDATNVGDEG GFAPNILENK EALELLKTAI AKAGYTDQVV IGMDVAASEF YRSGKYDLDF KSPDDPSRYI TPDQLADLYK SFVQNYPVVS IEDPFDQDDW GAWQKFTASA GIQVVGDDLT VTNPKRIAKA ASEKSCNCLL LKVNQIGSVT ESLQACKLAQ SNGWGVMVSH RSGETEDTFI ADLVVGLCTG QIKTGAPCRS ERLAKYNQIL RIEEELGSKA KFAGRSFRNP LA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses . May also function in the intravascular and pericellular fibrinolytic syst due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production . Reference: SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.Mol. Cell 50:919-930(2013). Function: nan