Alpha-2-HS-glycoprotein (AHSG) (Active), recombinant Bovine

Organism: Bos taurus (Bovine). Source: Mammalian cell. Expression Region: 19-359aa. Protein Length: Full Length of Mature Protein. Tag Info: Tag free. Target Protein Sequence: IPLDPVAGYK EPACDDPDTE QAALAAVDYI NKHLPRGYKH TLNQIDSVKV WPRRPTGEVY DIEIDTLETT CHVLDPTPLA NCSVRQQTQH AVEGDCDIHV LKQDGQFSVL FTKCDSSPDS AEDVRKLCPD CPLLAPLNDS RVVHAVEVAL ATFNAESNGS YLQLVEISRA QFVPLPVSVS VEFAVAATDC IAKEVVDPTK CNLLAEKQYG FCKGSVIQKA LGGEDVRVTC TLFQTQPVIP QPQPDGAEAE APSAVPDAAG PTPSAAGPPV ASVVVGPSVV AVPLPLHRAH YDLRHTFSGV ASVESSSGEA FHVGKTPIVG QPSIPGGPVR LCPGRIRYFK I. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: =0.5EU/mg by the LAL method. Biological Activity: Measured in a cell proliferation assay using B16-F1 cells. Recombinant Bovine Fetuin A stimulates adhesion of B16-F1 cells. The ED50 for this effect is =100 µg/mL. Optimal concentration depends on cell type as well as the application or research objectives. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered solution containing 10 mM PB, pH 7.4. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: n/a. Reference: n/a. Function: nan