Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A), partial, human, r

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 93-535aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: LLKELTSKKS LQVPSIYYHL PHLLKNEGSL QPAVQIGNGR TGVSIVMGIP TVKREVKSYL IETLHSLIDN LYPEEKLDCV IVVFIGETDI DYVHGVVANL EKEFSKEISS GLVEVISPPE SYYPDLTNLK ETFGDSKERV RWRTKQNLDY CFLMMYAQEK GIYYIQLEDD IIVKQNYFNT IKNFALQLSS EEWMILEFSQ LGFIGKMFQA PDLTLIVEFI FMFYKEKPID WLLDHILWVK VCNPEKDAKH CDRQKANLRI RFRPSLFQHV GLHSSLSGKI QKLTDKDYMK PLLLKIHVNP PAEVSTSLKV YQGHTLEKTY MGEDFFWAIT PIAGDYILFK FDKPVNVESY LFHSGNQEHP GDILLNTTVE VLPFKSEGLE ISKETKDKRL EDGYFRIGKF ENGVAEGMVD PSLNPISAFR LSVIQNSAVW AILNEIHIKK ATN. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. Reference: "Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase." Yoshida A., Minowa M.T., Takamatsu S., Hara T., Oguri S., Ikenaga H., Takeuchi M. Glycobiology 9:303-310(1999). Function: nan