Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (MGAT1), partial, rabbit, rec

Organism: Oryctolagus cuniculus (Rabbit). Source: E.coli. Expression Region: 30-447aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: TRPVPSRLPS DNALDDDPAS LTREVIRLAQ DAEVELERQR GLLQQIREHH ALWSQRWKVP TAAPPAQPHV PVTPPPAVIP ILVIACDRST VRRCLDKLLH YRPSAELFPI IVSQDCGHEE TAQVIASYGS AVTHIRQPDL SNIAVQPDHR KFQGYYKIAR HYRWALGQIF HNFNYPAAVV VEDDLEVAPD FFEYFQATYP LLKADPSLWC VSAWNDNGKE QMVDSSKPEL LYRTDFFPGL GWLLLAELWA ELEPKWPKAF WDDWMRRPEQ RKGRACVRPE ISRTMTFGRK GVSHGQFFDQ HLKFIKLNQQ FVPFTQLDLS YLQQEAYDRD FLARVYGAPQ LQVEKVRTND RKELGEVRVQ YTGRDSFKAF AKALGVMDDL KSGVPRAGYR GIVTFLFRGR RVHLAPPQTW DGYDPSWT. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans. Reference: "Molecular cloning and expression of cDNA encoding the enzyme that controls conversion of high-mannose to hybrid and complex N-glycans: UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I." Sarkar M., Hull E., Nishikawa Y., Simpson R.J., Moritz R.L., Dunn R., Schachter H. Proc. Natl. Acad. Sci. U.S.A. 88:234-238(1991). Function: nan