Alkaline phosphatase, tissue-nonspecific isozyme (Alpl),partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 20-503aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: PEKERDPSYW RQQAQETLKN ALKLQKLNTN VAKNVIMFLG DGMGVSTVTA ARILKGQLHH NTGEETRLEM DKFPFVALSK TYNTNAQVPD SAGTATAYLC GVKANEGTVG VSAATERTRC NTTQGNEVTS ILRWAKDAGK SVGIVTTTRV NHATPSAAYA HSADRDWYSD NEMPPEALSQ GCKDIAYQLM HNIKDIDVIM GGGRKYMYPK NRTDVEYELD EKARGTRLDG LDLISIWKSF KPRHKHSHYV WNRTELLALD PSRVDYLLGL FEPGDMQYEL NRNNLTDPSL SEMVEVALRI LTKNLKGFFL LVEGGRIDHG HHEGKAKQAL HEAVEMDQAI GKAGAMTSQK DTLTVVTADH SHVFTFGGYT PRGNSIFGLA PMVSDTDKKP FTAILYGNGP GYKVVDGERE NVSMVDYAHN NYQAQSAVPL RHETHGGEDV AVFAKGPMAH LLHGVHEQNY IPHVMAYASC IGANLDHCAW AGSG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: Induction of alkaline phosphatase in mouse L cells by overexpression of the catalytic subunit of cAMP-dependent protein kinase.Brown N.A., Stofko R.E., Uhler M.D.J. Biol. Chem. 265:13181-13189(1990). Function: This isozyme may play a role in skeletal mineralization.