Alanine aminotransferase 1 (Gpt), mouse, recombinant

Organism: Mus musculus (Mouse). Source: Yeast. Expression Region: 2-496aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ASQRNDRIQA SRNGLKGKVL TLDTMNPCVR RVEYAVRGPI VQRALELEQE LRQGVKKPFT EVIRANIGDA QAMGQRPITF FRQVLALCVY PNLLSSPDFP EDAKRRAERI LQACGGHSLG AYSISSGIQP IREDVAQYIE RRDGGIPADP NNIFLSTGAS DAIVTMLKLL VAGEGRARTG VLIPIPQYPL YSAALAELDA VQVDYYLDEE RAWALDIAEL RRALCQARDR CCPRVLCVIN PGNPTGQVQT RECIEAVIRF AFEEGLFLMA DEVYQDNVYA EGSQFHSFKK VLTEMGPPYA TQQELASFHS VSKGYMGECG FRGGYVEVVN MDAEVQKQMA KLMSVRLCPP VPGQALMGMV VSPPTPSEPS FKQFQAERQE VLAELAAKAK LTEQVFNEAP GIRCNPVQGA MYSFPQIQLP LKAVQRAQDL GLAPDMFFCL CLLEETGICV VPGSGFGQQE GTYHFRMTIL PPMEKLRVLL EKLRHFHAKF THEYS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). Reference: "A tissue-specific atlas of mouse protein phosphorylation and expression."Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.Cell 143:1174-1189(2010). Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).