Agglutinin-like protein 3 (ALS3), partial, Candida albicans, recombinant

Organism: Candida albicans (Yeast). Source: E.coli. Expression Region: 918-1119aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-B2M-tagged and C-terminal Myc-tagged. Target Protein Sequence: VSVETESSTV TTAQTNPSVP TTESEVVFTT KGNNENGPYE SPSTNVKSSM DENSEFTTST AASTSTDIEN ETIATTGSVE ASSPIISSSA DETTTVTTTA ESTSVIEQPT NNNGGGKAPS ATSSPSTTTT ANNDSVITGT TSTNQSQSQS QYNSDTQQTT LSQQMTSSLV SLHMLTTFDG SGSVIQHSTW LCGLITLLSL FI. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the pathogenesis of C.albicans infections. Necessary for C.albicans to bind to N-cadherin on endothelial cells and E-cadherin on oral epithelial cells and subsequent endocytosis by these cells. Reference: "The peptide-binding cavity is essential for Als3-mediated adhesion of Candida albicans to human cells." Lin J., Oh S.H., Jones R., Garnett J.A., Salgado P.S., Rusnakova S., Matthews S.J., Hoyer L.L., Cota E. J. Biol. Chem. 289:18401-18412(2014). Function: Cell surface adhesion protein which mediates both yeast-to-host tissue adherence and yeast aggregation. Plays an important role in the biofilm formation and pathogenesis of C.albicans infections. Necessary for C.albicans to bind to N-cadherin on endothelial cells and E-cadherin on oral epithelial cells and subsequent endocytosis by these cells. During disseminated infection, mediates initial trafficking to the brain and renal cortex and contributes to fungal persistence in the kidneys.