AGE-BSA Reagent Set

Advanced glycation end products (AGEs) are formed from the non-enzymatic reaction of amino groups with reducing sugars. AGEs have been implicated in several diseases, such as diabetes mellitus, non-diabetic nephropathy, macrovascular disease, Alzheimer's disease, cataracts, and aging. AGE receptors, such as the receptor for AGEs (RAGE), mediate biological responses to AGEs, including endocytic uptake and degradation and induction of cytokines and growth factors. AGE-BSA has been used as a standard for AGEs in ELISAs and to determine the effects of AGEs on cells in culture. AGE-BSA was produced by incubating BSA with glucose, followed by extensive dialysis. The AGE-BSA and BSA control were prepared at the same time with the same lot of BSA under sterile conditions, then filtered and aliquoted into sterile vials and lyophilized.