ADP/ATP translocase 1 (SLC25A4), recombinant human

Organism: Homo sapiens (Human). Source: in vitro E.coli expression system. Expression Region: 2-298aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: GDHAWSFLKD FLAGGVAAAV SKTAVAPIER VKLLLQVQHA SKQISAEKQY KGIIDCVVRI PKEQGFLSFW RGNLANVIRY FPTQALNFAF KDKYKQLFLG GVDRHKQFWR YFAGNLASGG AAGATSLCFV YPLDFARTRL AADVGKGAAQ REFHGLGDCI IKIFKSDGLR GLYQGFNVSV QGIIIYRAAY FGVYDTAKGM LPDPKNVHIF VSWMIAQSVT AVAGLVSYPF DTVRRRMMMQ SGRKGADIMY TGTVDCWRKI AKDEGAKAFF KGAWSNVLRG MGGAFVLVLY DEIKKYV. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. Relevance: ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell. Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane. In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity. Reference: n/a. Function: nan