Adhesion G-protein coupled receptor G6 (ADGRG6), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 38-437aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: CANCRVVLSN PSGTFTSPCY PNDYPNSQAC MWTLRAPTGY IIQITFNDFD IEEAPNCIYD SLSLDNGESQ TKFCGATAKG LSFNSSANEM HVSFSSDFSI QKKGFNASYI RVAVSLRNQK VILPQTSDAY QVSVAKSISI PELSAFTLCF EATKVGHEDS DWTAFSYSNA SFTQLLSFGK AKSGYFLSIS DSKCLLNNAL PVKEKEDIFA ESFEQLCLVW NNSLGSIGVN FKRNYETVPC DSTISKVIPG NGKLLLGSNQ NEIVSLKGDI YNFRLWNFTM NAKILSNLSC NVKGNVVDWQ NDFWNIPNLA LKAESNLSCG SYLIPLPAAE LASCADLGTL CQATVNSPST TPPTVTTNMP VTNRIDKQRN DGIIYRISVV IQNILRHPEV KVQSKVAEWL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: G-protein coupled receptor which is activated by type IV collagen, a major constituent of the basement membrane. Couples to G(i)-proteins as well as G(s)-proteins. Essential for normal differentiation of promyelinating Schwann cells and for normal myelination of axons. Regulates neural, cardiac and ear development via G-protein- and/or N-terminus-dependent signaling. May act as a receptor for PRNP which may promote myelin homeostasis. Reference: "Mutations of GPR126 are responsible for severe arthrogryposis multiplex congenita." Ravenscroft G., Nolent F., Rajagopalan S., Meireles A.M., Paavola K.J., Gaillard D., Alanio E., Buckland M., Arbuckle S., Krivanek M., Maluenda J., Pannell S., Gooding R., Ong R.W., Allcock R.J., Carvalho E.D., Carvalho M.D., Kok F. Laing N.G. Am. J. Hum. Genet. 96:955-961(2015). Function: nan