Adenomatous polyposis coli protein (APC), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 960-1337aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: SNDSLNSVSS SDGYGKRGQM KPSIESYSED DESKFCSYGQ YPADLAHKIH SANHMDDNDG ELDTPINYSL KYSDEQLNSG RQSPSQNERW ARPKHIIEDE IKQSEQRQSR NQSTTYPVYT ESTDDKHLKF QPHFGQQECV SPYRSRGANG SETNRVGSNH GINQNVSQSL CQEDDYEDDK PTNYSERYSE EEQHEEEERP TNYSIKYNEE KRHVDQPIDY SLKYATDIPS SQKQSFSFSK SSSGQSSKTE HMSSSSENTS TPSSNAKRQN QLHPSSAQSR SGQPQKAATC KVSSINQETI QTYCVEDTPI CFSRCSSLSS LSSAEDEIGC NQTTQEADSA NTLQIAEIKE KIGTRSAEDP VSEVPAVSQH PRTKSSRL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Tumor suppressor. Promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. APC activity is correlated with its phosphorylation state. Activates the GEF activity of SPATA13 and ARHGEF4. Plays a role in hepatocyte growth factor (HGF)-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization. Reference: "ErbB2 receptor controls microtubule capture by recruiting ACF7 to the plasma membrane of migrating cells." Zaoui K., Benseddik K., Daou P., Salaun D., Badache A. Proc. Natl. Acad. Sci. U.S.A. 107:18517-18522(2010). Function: nan