Acyl-CoA synthetase family member 2, mitochondrial (ACSF2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 42-615aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LSSREVDRMV STPIGGLSYV QGCTKKHLNS KTVGQCLETT AQRVPEREAL VVLHEDVRLT FAQLKEEVDK AASGLLSIGL CKGDRLGMWG PNSYAWVLMQ LATAQAGIIL VSVNPAYQAM ELEYVLKKVG CKALVFPKQF KTQQYYNVLK QICPEVENAQ PGALKSQRLP DLTTVISVDA PLPGTLLLDE VVAAGSTRQH LDQLQYNQQF LSCHDPINIQ FTSGTTGSPK GATLSHYNIV NNSNILGERL KLHEKTPEQL RMILPNPLYH CLGSVAGTMM CLMYGATLIL ASPIFNGKKA LEAISRERGT FLYGTPTMFV DILNQPDFSS YDISTMCGGV IAGSPAPPEL IRAIINKINM KDLVVAYGTT ENSPVTFAHF PEDTVEQKAE SVGRIMPHTE ARIMNMEAGT LAKLNTPGEL CIRGYCVMLG YWGEPQKTEE AVDQDKWYWT GDVATMNEQG FCKIVGRSKD MIIRGGENIY PAELEDFFHT HPKVQEVQVV GVKDDRMGEE ICACIRLKDG EETTVEEIKA FCKGKISHFK IPKYIVFVTN YPLTISGKIQ KFKLREQMER HLNL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation. Reference: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. Gray A.M.Genome Res. 13:2265-2270(2003). Function: Acyl-CoA synthases catalyze the initial reaction in fatty acid metabolism, by forming a thioester with CoA. Has some preference toward medium-chain substrates. Plays a role in adipocyte differentiation.